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A comparison of methods employed to evaluate antioxidant capabilities Biol. Res.
PEREZ,DRUSO D; LEIGHTON,FEDERICO; ASPEE,ALEXIS; ALIAGA,CAROLINA; LISSI,EDUARDO.
Thrce ditferent methodologies frequently employed to evaluate the indexes that report the antioxidant capabilities of pure compounds and/or complex mixtures of antioxidants are applied to a series of mono- and polyphenols, as well as to two wine (red and white) samples. These methodologies are based on the bleaching of a stable radical, the effect of the additive upon luminol chemiluminescence induced by peroxyl radicals, and the effect of the additive upon the bleaching of the fluorescence from a dye molecule. Widely ditferent responses are obtained from the different methodologies. These differences are interpreted in terms of the different factors (stoichiametric factors and/or reactivities) that determines the indexes evaluated by these different...
Tipo: Journal article Palavras-chave: ABTS radical bleaching; Antioxidants; C-phycocyanin bleaching; Total antioxidant reactivity; Total reactive antioxidant potential.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200006
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Actividad antioxidante de algas y plantas marinas de la plataforma insular cubana OceanDocs
Valdés, O.; Hernádez, Y.; Fernández, M.D.; Hernández, I.; Rodriguez, M.; Cano, M.; Laguna, A.; Díaz, C.; Cabrera, B..
En el presente trabajo se estudia la capacidad antioxidante de varios extractos, acuosos e hidro-alcohólicos obtenidos a partir de algas y plantas marinas de la plataforma insular cubana. A todos los extractos se les determinó su contenido de polifenoles, proteínas, lípidos y azúcares totales, así como la actividad Superóxido Dismutasa (SOD). Todos los extractos evaluados mostraron una buena actividad antioxidante, con una CE50 inferior de 0,4 mg.ml-1. El contenido de polifenoles en los extractos de Ulva fasciata es menor el de las otras especies, aunque tanto el extracto hidro-alcohólico como el del alga seca e irradiada de dicha especie presentan una buena actividad antioxidante. El extracto hidro-alcohólico de Thalassia testudinum presenta el mayor...
Tipo: Journal Contribution Palavras-chave: Proteins; Antioxidants; Proteins; Antioxidants; Http://aims.fao.org/aos/agrovoc/c_6259; Http://aims.fao.org/aos/agrovoc/c_511.
Ano: 2008 URL: http://hdl.handle.net/1834/2820
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Actividad antioxidante en extractos de algas marinas OceanDocs
Fernández, M.D.; Hernández, I.; Aneiros, A.; Tello, J..
Entre otras múltiples reacciones, los radicales libres pueden interactuar con ácidos grasos de las membranas celulares y causar la destrucción oxidativa de las mismas; a este proceso es al que se le denomina lipoperoxidación (Echeverry, 1998). Como defensas frente a las agresiones de los radicales libres se presentan una serie de sistemas enzimáticos entre los que destacan la superóxido dismutasa y la catalasa. En los organismos marinos se han encontrado compuestos con actividad antioxidantes fundamentalmente en algunas especies de algas. En este trabajo se realizó la evaluación del efecto antioxidante de los extractos de 6 algas mediante los métodos de inhibición de la peroxidación lipídica, determinación de la actividad mimética superóxido dismutasa...
Tipo: Other Palavras-chave: Antioxidants; Algae; Experimental research; Antioxidants; Algae; Http://aims.fao.org/aos/agrovoc/c_511; Http://aims.fao.org/aos/agrovoc/c_258.
Ano: 2007 URL: http://hdl.handle.net/1834/2604
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Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract BABT
Naine,S. Jemimah; Devi,C. Subathra; Mohanasrinivasan,V.; B,Vaishnavi.
The main aim of the study was to evaluate the bioactive properties of ethyl acetate crude extract of Streptomyces parvulus VITJS11 with a view to assess their therapeutic potential. The biological activity of ethyl acetate extract was tested against fungal and bacterial pathogens. The free radical scavenging potential of the crude extract was determined by DPPH assay. The chemo preventive properties of S. parvulus VITJS11 ethyl acetate extract was examined by MTT assay on HepG2 cells. The morphological, physiological and the biochemical properties of the strain S. parvulus VITJS11 was confirmed by conventional methods. Genotypic characterization was done using 16S r-DNA partial gene amplification and sequencing. The authenticity of the crude chemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Marine actinomycetes; Streptomyces parvulusVITJS11; Antimicrobial activity; Antioxidants; Cytotoxicity; Bioactive compounds.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200198
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Anti-obesity Effects of the Administration of Tournefortia paniculata Cham Extract on Wistar Rats Subjected to a Hypercaloric Diet BABT
Simão,Anderson Assaid; Ramos,Vinicius de Oliveira; Corrêa,Angelita Duarte; Sousa,Raimundo Vicente de; Marcussi,Silvana.
The aim of this study was to evaluate the therapeutic and toxicologic effects of the administration of the powered vegetable extract and aqueous extract of Tournefortia paniculata leaves on Wistar rats, subjected to a hypercaloric diet for 42 days. The rats were divided into five groups and were given the following treatments by gavage: T0 (control) - 1.0 mL water day-1; T1 - aqueous extract containing 14 mg phenolic compounds kg-1 rat day-1; T2 - 14 mg quercetin kg-1 rat day-1; T3 - 50 mg powered vegetable extract from T. paniculata leaves (PVE) kg-1 rat day-1 and T4 - 100 mg PVE kg-1 rat day-1. The treatments did not significantly alter the weight, but were effective in reducing liver fat, glucose and serum triglycerides. The treatment T1 reduced food...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Obesity; Medicinal plants; Pharmacology; Antioxidants; Genotoxicity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400494
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ANTIOXIDANT ACTIVITY DURING STORAGE OF APPLES SUBJECTED TO IRRADIATION Ciência e Agrotecnologia
Fante,Camila Argenta; Elias,Heloísa Helena de Siqueira; Henrique,Paôla de Castro; Boas,Ana Carolina Vilas; Lima,Luiz Carlos de Oliveira.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Postharvest; Storage; Antioxidants; Food quality.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000300269
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Antioxidant activity of bee products added to water in tebuconazole-exposed fish Neotropical Ichthyology
Ferreira,Daiane; Unfer,Taís Cristina; Rocha,Hélio Carlos; Kreutz,Luiz Carlos; Koakoski,Gessi; Barcellos,Leonardo José Gil.
An experiment was conducted to evaluate the potential of honey, propolis, and bee pollen for the reversal of lipid peroxidation induced by tebuconazole (TEB) in South American catfish (Rhamdia quelen), in which the concentration of thiobarbituric acid reactive substances (TBARS), the activity of the antioxidant enzyme glutathione-S-transferase (GST) and the concentrations of non-enzymatic antioxidants, reduced glutathione (GSH), ascorbic acid, and non-protein thiols were assessed. Honey (0.125 g L-1) and bee pollen (0.05 g L-1) added to the water reverse the production of TBARS induced by TEB, while propolis demonstrated a pro-oxidant effect, inducing an increase in TBARS production. The data presented herein suggest that the addition of water to honey and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Bee pollen; Honey; Lipid peroxidation; Propolis; Tebuconazole.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000100021
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Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro Arq. Bras. Med. Vet. Zootec.
Sollecito,N.V.; Pereira,E.C.M.; Grázia,J.G.V.; Neves,B.P.; Couto,B.V.R.; Andrade,V.B.; Miranda,M.S.; Silva,J.K.R.; Borges,A.M..
ABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Apoptosis; Lippia origanoides; In vitro embryo production.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300723
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Antioxidant, analgesic and anti-inflammatory effects of lavender essential oil Anais da ABC (AABC)
SILVA,GABRIELA L. DA; LUFT,CAROLINA; LUNARDELLI,ADROALDO; AMARAL,ROBSON H.; MELO,DENIZAR A. DA SILVA; DONADIO,MÁRCIO V.F.; NUNES,FERNANDA B.; AZAMBUJA,MARCOS S. DE; SANTANA,JOÃO C.; MORAES,CRISTINA M.B.; MELLO,RICARDO O.; CASSEL,EDUARDO; PEREIRA,MARCOS AURÉLIO DE ALMEIDA; OLIVEIRA,JARBAS R. DE.
Several studies have investigated the antinociceptive, immunomodulatory and anti-inflammatory properties of compounds found in the lavender essential oil (LEO), however to date, there is still lack of substantial data. The objective of this study was to assess the antioxidant, anti-inflammatory and antinociceptive effects of lavender essential oil. The 1,1-diphenyl-2-picrylhydrazyl radical decolorization assay was used for antioxidant activity evaluation. The anti-inflammatory activity was tested using two models of acute inflammation: carrageenan-induced pleurisy and croton oil-induced ear edema. The antinociceptive activity was tested using the pain model induced by formalin. LEO has antioxidant activity, which is dose-dependent response. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Inflammation; Lavender; Nociception.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301397
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ANTIOXIDANT CAPACITY OF PEQUI (Caryocar brasiliense Camb.) PULP IS PRESERVED BY FREEZE-DRYING AND LIGHT-RESISTANT PACKAGING Rev. Bras. Frutic.
NASCIMENTO,NARA RÚBIA RODRIGUES DO; ALVES,ALINE MEDEIROS; SILVA,MARA REIS; NAVES,MARIA MARGARETH VELOSO.
ABSTRACT Pequi (Caryocar brasiliense Camb.) pulp is rich in bioactive compounds. However, seasonality and perishability limit its availability in the market. This work aimed to determine the physicochemical characteristics, including phenolic compounds and carotenoid concentrations, as well as the antioxidant capacity of lyophilized pequi pulp during storage in various packaging materials for 180 days. Pequi fruits were pulped, ground, freeze-dried and then vacuum packaged in transparent polyethylene (TP), polyethylene coated by aluminum foil (PA) and laminated foil (LA), respectively. The samples were stored under controlled temperature, humidity and luminosity. Proximate composition, phenolic compounds and carotenoids concentrations, and antioxidant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Carotenoids; Caryocar brasiliense; Cerrado; Pequi.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000100901
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Antioxidant potential of various solvent extract from Morus alba fruits and its major polyphenols composition Ciência Rural
Kobus-Cisowska,Joanna; Szczepaniak,Oskar; Szymanowska-Powałowska,Daria; Piechocka,Justyna; Szulc,Piotr; Dziedziński,Marcin.
ABSTRACT: Extraction conditions are an important factor in the process of obtaining bioactive compounds from plant matrix. These compounds differ structurally. Structures of phyto-compounds and their interactions with other food ingredient are not fully known, while these two aspects should play a significant role in extrahents choice and determination of extraction process conditions. Mulberry (Morus alba) is a plant growing in Asia, which fruits are rich in bioactive ingredients and high anti-oxidative potential. In our study we analyzed mulberry fruits extracts differing in the extra hent applied: acetone, methanol, ethanol and water. All tested extracts possessed rich polyphenolic composition and radical scavenging ability. The significant differences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morus alba; Antioxidants; Phenolic acids; Flavonoids.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000100751
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Ascorbate protects (+)-catechin from oxidation both in a pure chemical system and human plasma Biol. Res.
LOTITO,SILVINA B; FRAGA,CESAR G.
We evaluated the interaction between ascorbic acid (AA) and (+)-catechin (CTCH) in potassium phosphate solution, pH 7.4 (PPS) and in human plasma. In both systems, the oxidation was started by adding 2,2’-azobis-(2-amidinopropane) clorhidrate (AAPH). The concentrations of AA and CTCH were determined by HPLC using electrochemical detection. In PPS, CTCH was oxidized by AAPH (50 muM), in either the absence or presence of different initial concentrations of AA (25-200 muM). In the presence of AA, CTCH depletion was delayed, an effect that was dependent upon the initial concentration of AA. When 100 muM AA was added after the oxidation had begun, CTCH depletion was arrested for 30 min. The kinetics of AA oxidation by AAPH was also characterized in PPS. AA (100...
Tipo: Journal article Palavras-chave: Catechins; Antioxidants; Free radicals; Procyanidins.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200015
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Assessment of antioxidants status and superoxide dismutase activity in HIV-infected children BJID
Pugliese,Camila; Patin,Rose Vega; Palchetti,Cecilia Zanin; Claudio,Cristiane Chiantelli; Gouvêa,Aída de Fátima Thomé Barbosa; Succi,Regina Célia de Menezes; Amancio,Olga Maria Silverio; Cozzolino,Silvia Maria Franciscato; Oliveira,Fernanda Luisa Ceragioli.
Objective:This study aims to assess the nutritional status of selenium, copper and zinc; and also the erythrocyte superoxide dismutase activity of HIV-infected children compared to a control group.Methods:A cross-sectional study was carried out with prepubertal HIV-infected children (n = 51) and their healthy siblings (n = 32). All biochemical measurements including plasma selenium, serum copper levels, serum and erythrocyte zinc levels and erythrocyte super-oxide dismutase activity were evaluated according to dietary, clinical and biochemical parameters.Results:Compared to the control group, the HIV-infected children had lower z-score values for height-for-age (p = 0.0006), higher prevalence of stunting (11.8%) (p = 0.047), lower selenium levels (p =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; HIV and nutrition; Oxidative stress; Trace elements.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500481
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Avenanthramides and nutritional components of four mexican oat (Avena sativa L.) varieties Agrociencia
Ortiz-Robledo,Faviola; Villanueva-Fierro,Ignacio; Oomah,B. Dave; Lares-Asef,Ismael; Proal-Nájera,J. Bernardo; Návar-Chaidez,J. Jesús.
Oat (Avena sativa L.) is a cereal grain, its seeds are rich in nutrients, is consumed as whole grain with health benefits fot humans due to its antioxidants, such as the avenanthtamides (Avns). The contents of Avns 2c, 2f and 2p were evaluated by HPLC in groats and hulls of oat varieties Chihuahua, Cuauhtemoc, Raima and Avemex cultivated in Durango, México. The association of these Avns with protein, fat, crude fiber and ash of oat was also investigated. A 4x2 bifactorial design was used, with the number of varieties and groats ot hulls as factors. Avns contents differed significantly (p<0.05) among oat varieties, groats and hulls. Avemex groats had the highest concentrations of Avns: 3.6±0.7, 3.0±0.5 and 3.3±0.6 mg kg-1 fot 2c, 2f and 2p Avns. Avemex...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Groats; Hulls; Oat; Polyphenols.
Ano: 2013 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952013000300002
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Bioactive compounds and antioxidant activity three fruit species from the Brazilian Cerrado Rev. Bras. Frutic.
Moreira-Araújo,Regilda Saraiva dos Reis; Barros,Nara Vanessa dos Anjos; Porto,Rayssa Gabriela Costa Lima; Brandão,Amanda de Castro Amorim Serpa; Lima,Alessandro de; Fett,Roseane.
Abstract The aim of this study was to determine the content of bioactive compounds and antioxidant activity present in three fruit species from the Brazilian Cerrado: carnauba (Copernicia prunifera (Mill.) H.E. Moore), murici (Byrsonima crassifolia L. Rich)and oiti (Licania tomentosa (Benth) Fritsch). Among analyzed fruits, oiti showed the highest total phenolic content (1236.42 ± 34.06 mg GAE 100 g-1) followed by murici (468.90 ± 27.30 mg GAE 100 g-1) and carnauba (314.44 ± 9.50 mg GAE 100 g-1). Regarding the antioxidant activity, murici showed 4350.31 ± 1.85 µmol TEAC.100 g-1 and oiti showed 14721.69 ± 0.85 µmol TEAC.100 g-1. In addition, high content of anthocyanins was verified in carnauba (9.35 ± 0.00 mg-cy-3-glu.100 g-1), as well as carotenoids...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Anthocyanins; Carotenoids; Phenolic compounds; Cerrado.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000300901
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Bioactive micro-constituents of ackee arilli (Blighia sapida K.D. Koenig) Anais da ABC (AABC)
GRANDE-TOVAR,CARLOS D.; JOHANNES,DELGADO-OSPINA; PUERTA,LUISA F.; RODRÍGUEZ,GLORIA C.; SACCHETTI,GIAMPIERO; PAPARELLA,ANTONELLO; CHAVES-LÓPEZ,CLEMENCIA.
Abstract: Ackee (Blighia sapida K. D. Koenig) is an exotic fruit widely consumed in the Caribbean countries. While there is extensive research on the presence of hypoglycin A, other bioactive compounds have not been studied. We identified and quantified the changes in bioactive molecules (total phenol, ascorbic acid, hypoglycin A, squalene, D: A-Friedooleanan-7-ol, (7.alpha.), and oleic acid), antioxidant potential, and volatile compounds during two stages of ripe. A clear reduction in hypoglycin A, ascorbic acid, and total polyphenols during the maturation process were observed. On the contrary, oleic acid, squalene, and D: A-Friedooleanan-7-ol, (7.alpha.) contents increased about 12, 12, and 13 times, respectively with advancing maturity. These bioactive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Friedooleanan; Hypoglycin; Polyphenols; Squalene.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500616
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Bioactive phytochemicals and antioxidant activity in fresh and dried lychee fractions1 Rev. Ciênc. Agron.
Queiroz,Estela de Rezende; Abreu,Celeste Maria Patto de; Oliveira,Kelly da Silva; Ramos,Vinicius de Oliveira; Fráguas,Rodrigo Martins.
Fruit of the lychee cv. Bengal are approximately 50% peel and seeds, which are discarded. These by-products have antioxidant compounds which are capable of blocking the harmful effects of free radicals in the body. Bioactive compounds (ascorbic acid, beta-carotene, lycopene and phenols) and antioxidant activity were evaluated in different extracts, both fresh and dried at 45 °C, of the skin, pulp and seeds of the lychee, which were subjected to principal component analysis to clarify which of the compounds are responsible for this activity. Principal component analysis explained 82.90% of the variance of the antioxidant profile of the lychee. The peel displayed higher levels of phenols, ascorbic acid, beta-carotene and antioxidant activity, while the seeds...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lychee; Antioxidants; Principal component analysis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000100163
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Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa Neotropical Ichthyology
Garcia,Cylene Zambrozi; Martinez,Claudia B. R..
Microcystins are secondary metabolites produced by different species of cyanobacteria, such as Microcystis aeruginosa (MA). In this study, the biochemical and genetic effects of lyophilized MA were evaluated in the neotropical fish Prochilodus lineatus exposed to 1 or 2 mg L-1 lyophilized MA (treated group) or only water (control group) in static toxicity tests for 24 and 96 h. The gills and liver were used in the analysis of biotransformation enzymes and antioxidant defenses, blood and gill cells in genetic analysis and in brain and muscle it was determined the activity of acetylcholinesterase (AChE). The results showed the biotransformation pathway activation due to the increase in hepatic CYP1A and in branchial and hepatic glutathione S-transferase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetylcholinesterase; Antioxidants; Biotransformation; Cyanotoxin; DNA damage.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300015
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Biological applications of honeys produced by Apis mellifera Biol. Res.
Montenegro,Gloria; Mejías,Enrique.
Honey is a natural product with many attributes that are useful for humans. The consumption of honey is increasing because of its beneficial biological properties, including antioxidant and antibacterial activities. Even though honey is produced worldwide, many variants of this product have not been studied and their biological potential for alternative uses has not been evaluated. Because of its varied endemic native flora, Chile produces several types of honey. It is likely that these apian products have important biological properties inherited from specific floral sources, but it is absolutely necessary to conduct further chemical analyses to identify and characterize these biological attributes. Sadly, the potential antimicrobial and antitumor...
Tipo: Journal article Palavras-chave: Honeys; Antioxidants; Natural Antibiotics; Environmental Bioindicator.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400005
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Biomass Accumulation, Photosynthetic Pigments, Osmotic Adjustments and Antioxidant Activities of Leymus chinensis in Response to BA, BR, and GA Planta Daninha
XUE-FENG,Z.; JUN,L.V.; SHAHID,M.; ANJUM,S.A.; NA-JIA,L.; XIU-JUAN,H.; YU,X.; XIAO,W.; SAN-GEN,W..
ABSTRACT: Abiotic stresses and poor biomass accumulation are chief constraints to accomplish potential yield in Leymus chinensis. An experiment was conducted to improve biomass and correlation of biomass accumulating attributes with photosynthetic pigments, osmotic substances and antioxidants under foliar application of different plant growth substances. The experiment was conducted at inner Mongolia Xilinguole, China, using a Randomized Complete Block Design and 5 replications. The treatments consisted of water (control); BA5 = application of BA (6-benzylaminopurine) at 5 mg L-1; BA25 = application of BA at 25 mg L-1; BA50 = application of BA at 50 mg L-1; BR0.02 = application of BR (brassinosteroid) at 0.02 g L-1; BR0.2 = application of BR at 0.2 mg L 1;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Reactive oxygen species; Phytoregulators.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100256
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